240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com
A DNA helicase that can improve specificity of isothermal amplification reactions and can unwind dsDNA, without a specific flap or overhang structure.
This includes duplex DNA and duplexes with 3′and 5′ ssDNA overhangs (1-3). References 2019-01-14 · FAQ: Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)? No, Tte UvrD Helicase should not be used with Colorimetric LAMP. As the UvrD acts on DNA substrates it hydrolyzes dATP, releasing an H+ with each hydrolysis much like a DNA polymerase incorporating dNTP. 2019-01-16 · Prepare a 20 µl reaction as follows: Tte UvrD Helicase 20 ng (1 μL) DNA up to 1 µg Isothermal Amplification Buffer (10X) 2 μL (1X) ATP (10 mM) 2 μL (1 mM) Nuclease-Free Water to 20 μL Incubation Temperature 65°C Incubation Time 10 min Incubate at 65°C for 10 minutes Stop reaction by heating to 80°C or addition of EDTA (to 10 mM) Tte UvrD Helicase Eliminates Non-Template Amplification in LAMP Reactions LAMP reactions were performed using the WarmStart ® LAMP Kit (DNA & RNA) ( NEB #E1700 2019-01-14 · FAQ: How do I use Tte UvrD Helicase for reducing non-template amplification in LAMP reactions We suggest adding 10 ng Tte UvrD Helicase to a 25 μl LAMP reaction using the WarmStart ® LAMP Kit ( NEB #E1700 ).
New England Biolabs GmbH Brüningstr. 50; Geb. B852 D-65926 Frankfurt am Main Tel: +49-69/305-23140 E-Mail: info.de@neb.com 5'TCGA3'3'AGCT5'Thermo Scientific TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzyme Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification. Tte-UvrD Helicase - 50 rxns: Amazon.com: Industrial & Scientific.
2 Feb 2017 of UvrD helicase and Mfd translocase in repair of UV-induced dam- 1 h at 37 ° C, and then with 12 μL of proteinase K (P8107S; NEB) for 1.5 h
Use the NEB LAMP Primer Design Tool. NEB's WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity Tte UvrD Helicase.
2019-01-16 · WarmStart LAMP Master Mix (E1700, 2X) 12.5 μL LAMP Fluorescent Dye (50X) 0.5 μL Primers (10X) 2.5 μL* Tte UvrD Helicase : 0.5 – 1 μL (10 – 20 ng) DNA or RNA sample
(1). EcoRI transcriptase in presence of UvrD helicase that unwinds the RNA-DNA hybrid enabling 6.6µM Helicase (BioHelix), 2.5µM Template, 7.5µM ET SSB (NEB). 6 Aug 2020 ATP, 1µM SYTO 9, 1x primer mix, 0.1ng/µl Tte UvrD Helicase (NEB, #M1202S), 0.05U/µl thermostable inorganic pyrophosphatase (NEB, as sizing marker (NEB). FIG. 14 . Real-time detection of an oral pathogen, T. denticola, by HDA method. HDA reaction was carried out using an UvrD helicase 25 May 2020 The superfamily I helicases, UvrD and Rep are similar in structure and RecA (1 μM; from NEB) and 2 mM ATP were used for RecA assembly 5 Mar 2021 LAMP reaction, namely Tte UvrD helicase, which prevents unspecific 0.1 ng/ µl Tte UvrD Helicase (NEB, #M1202S), 0.05 U/µl thermostable NEB® Golden Gate Assembly Kit (BsmBI-v2) · StickTogether™ DNA Ligase Buffer · NEBExpress MBP Tte-UvrD Helicase · Exo-CIP Rapid PCR Cleanup Kit LAMP primers? Use the NEB LAMP Primer Design Tool.
These enzymes have interesting properties and unique specificities that are not commercially available elsewhere at the quality that you would expect from NEB.
2019-01-16 · 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com
2019-01-16 · 240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com
A DNA helicase that can improve specificity of isothermal amplification reactions and can unwind dsDNA, without a specific flap or overhang structure. Without Tte UvrD Helicase (left), the positive reaction (+DNA) amplifies in ~8 minutes while the no-template control (NTC) amplifies in ~20 minutes. In the presence of 10 ng Tte UvrD Helicase (right), the positive reaction maintains its rapid amplification time with a slight reduction in total RFU, while the NTC reaction is completely suppressed. Are you doing COVID-19 related research?
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FAQ: Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding? No, Tte UvrD Helicase can utilize any dsDNA structure as a substrate for unwinding. This includes duplex DNA and duplexes with 3′and 5′ ssDNA overhangs (1-3). References Optimal activity is achieved with the recommended Isothermal Amplification Buffer supplemented with 1 mM ATP or in the WarmStart LAMP 2X Master Mix (NEB #E1700).
As the UvrD acts on DNA substrates it hydrolyzes dATP, releasing an H+ with each hydrolysis much like a DNA polymerase incorporating dNTP.
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Nt.BstNBI (NEB #R0607) • High purity, high quality nicking endonuclease • Glycerol-free • High conc. Helicase-dependent Amplification Tte UvrD Helicase (NEB #M1202) • Thermostable • Improves specifically of problematic fluorescent LAMP reactions • High conc.
enzymes were purchased from NEB (Ipswich, USA). polymerase (NEB), and 1 × Phusion HF Buffer). version containing a UvrD helicase domain, in Zymo-. 20 Aug 2010 As nonreplicative helicases, the roles of PcrA and UvrD in vivo are yet to be fully buffer of 1x NEB#1 for 10 min in a total volume of 20 μl.
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This intercalating dye enables real-time fluorescence measurement of a LAMP reaction.
Product Source Tte UvrD Helicase is isolated from a strain of E. coli which carries the cloned UvrD gene from Thermoanaerobacter tengcongensis. 2019-01-16 · WarmStart LAMP Master Mix (E1700, 2X) 12.5 μL LAMP Fluorescent Dye (50X) 0.5 μL Primers (10X) 2.5 μL* Tte UvrD Helicase : 0.5 – 1 μL (10 – 20 ng) DNA or RNA sample 2019-01-14 · FAQ: Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding? No, Tte UvrD Helicase can utilize any dsDNA structure as a substrate for unwinding. This includes duplex DNA and duplexes with 3′and 5′ ssDNA overhangs (1-3). References 2019-01-14 · FAQ: Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)? No, Tte UvrD Helicase should not be used with Colorimetric LAMP. As the UvrD acts on DNA substrates it hydrolyzes dATP, releasing an H+ with each hydrolysis much like a DNA polymerase incorporating dNTP.
240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com
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